Journal: Methods (San Diego, Calif.)

Article Title: Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology

doi: 10.1016/j.ymeth.2013.09.006

Figure Lengend Snippet: Flow chart of the CD45-based negative depletion and analysis pathway.

Article Snippet: The immunomagnetic labeling protocol involves two reagents: a CD45 tetrameric antibody complex (TAC) (Stem Cell Technologies) and dextran-coated magnetic nanoparticles (Stem Cell Technologies).

Techniques:

Journal: Methods (San Diego, Calif.)

Article Title: Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology

doi: 10.1016/j.ymeth.2013.09.006

Figure Lengend Snippet: Table of primary antibodies.

Article Snippet: The immunomagnetic labeling protocol involves two reagents: a CD45 tetrameric antibody complex (TAC) (Stem Cell Technologies) and dextran-coated magnetic nanoparticles (Stem Cell Technologies).

Techniques:

Journal: Methods (San Diego, Calif.)

Article Title: Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology

doi: 10.1016/j.ymeth.2013.09.006

Figure Lengend Snippet: List of CD45 antibody clones, fluoroprobes, and manufacturers tested.

Article Snippet: The immunomagnetic labeling protocol involves two reagents: a CD45 tetrameric antibody complex (TAC) (Stem Cell Technologies) and dextran-coated magnetic nanoparticles (Stem Cell Technologies).

Techniques: Clone Assay

Journal: Methods (San Diego, Calif.)

Article Title: Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology

doi: 10.1016/j.ymeth.2013.09.006

Figure Lengend Snippet: (A) Histograms of various anti-CD45 antibody clones, based on FCM analysis of peripheral blood leukocytes, both before (blue) and after (red) immunomagnetic labeling with anti-CD45-TAC/magnetic nanoparticles. Unstained controls are shown in orange. (B) Bar graph comparing the percentage of cells positive for each clone of anti-CD45 antibody, based on FCM analysis, both before and after immunomagnetic labeling.

Article Snippet: The immunomagnetic labeling protocol involves two reagents: a CD45 tetrameric antibody complex (TAC) (Stem Cell Technologies) and dextran-coated magnetic nanoparticles (Stem Cell Technologies).

Techniques: Clone Assay, Labeling

Journal: Methods (San Diego, Calif.)

Article Title: Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology

doi: 10.1016/j.ymeth.2013.09.006

Figure Lengend Snippet: Four-color images of a cytospin stained for DAPI, CK, CD45, and EpCAM of (a–e) leukocytes, (f–j) breast cancer cell line MCF7, and (k–o) an enriched peripheral blood sample from a metastatic breast cancer patient. The first column is a combined image of the four colors and the remaining columns (left to right) are DAPI, anti-CK-AF488, anti-CD45-AF594, and anti-EpCAM-AF647, respectively. For the patient sample, Cell #1 is CK+CD45+EpCAM− and Cell #2 is CK−CD45+EpCAM+.

Article Snippet: The immunomagnetic labeling protocol involves two reagents: a CD45 tetrameric antibody complex (TAC) (Stem Cell Technologies) and dextran-coated magnetic nanoparticles (Stem Cell Technologies).

Techniques: Staining

Journal: Methods (San Diego, Calif.)

Article Title: Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology

doi: 10.1016/j.ymeth.2013.09.006

Figure Lengend Snippet: (A) Four-color images of a cytospin stained for DAPI, CK, CD45, and VIM of (a–e) leukocytes, and (f–j) an enriched peripheral blood sample from a cancer patient. The columns (left to right) are DAPI, anti-CK-AF488, anti-CD45-AF594, anti-VIM-AF647, and a combined image of the four colors, respectively. (B) Four-color image of a cytospin stained for DAPI, CK, Vim, and N-CAD of an enriched peripheral blood sample from a cancer patient. (a) DAPI; (b) anti-CK-AF488; (c) anti-Vim-AF555; (d) anti-N-CAD-AF633 and (e) combined image of the four colors. The cells in the yellow boxes are CK+Vim+N-CAD+. Reproduced with permission from Balasubramanian et al. [15].

Article Snippet: The immunomagnetic labeling protocol involves two reagents: a CD45 tetrameric antibody complex (TAC) (Stem Cell Technologies) and dextran-coated magnetic nanoparticles (Stem Cell Technologies).

Techniques: Staining

Journal: Methods (San Diego, Calif.)

Article Title: Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology

doi: 10.1016/j.ymeth.2013.09.006

Figure Lengend Snippet: Four-color image of a cytospin stained for DAPI, CK, CD45, and HER2 of an enriched peripheral blood sample from a metastatic breast cancer patient. (a) DAPI; (b) anti-CK-AF488; (c) anti-CD45-AF594; (d) anti-HER2-AF647 and (e) combined image of the four colors. The cell in this figure is CK+CD45−HER2+, a HER2-expressing CTC.

Article Snippet: The immunomagnetic labeling protocol involves two reagents: a CD45 tetrameric antibody complex (TAC) (Stem Cell Technologies) and dextran-coated magnetic nanoparticles (Stem Cell Technologies).

Techniques: Staining, Expressing

Journal: Microbiology Spectrum

Article Title: A challenging STEC strain isolation from patients’ stools: an O166:H15 STEC strain with the stx2 gene

doi: 10.1128/spectrum.00098-24

Figure Lengend Snippet: Steps followed for STEC O166:H15 isolation. TSB: trypticase soy broth; PCR: polymerase chain reaction; MAC: MacConkey agar; IMS: immunomagnetic separation; BAP: sheep blood agar; STEC: Shiga toxin-producing E. coli .

Article Snippet: In IMS, a pool of immunomagnetic beads (RapidCheck CONFIRM STEC IMS, Romer Lab Division Holdings GmbH, Getzersdorf, Austria) that target O26, O45, O103, O111, O121, and O145 STEC are used to separate these STEC from the other background bacteria in a sample.

Techniques: Isolation, Polymerase Chain Reaction, Immunomagnetic Separation